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Image Search Results
Journal: bioRxiv
Article Title: Direct measurement of RNA Polymerase II hypertranscription in cancer FFPE samples
doi: 10.1101/2024.02.28.582647
Figure Lengend Snippet: Pathology classification, age and sex were provided by the vendor (BioChain). Each image spans the width of a standard charged microscope slide, where the tissue is visible under the paraffin skin. On-slide RNAPII-Ser5p FFPE-CUTAC was applied to slides in parallel, using a total of four slides each for 100 separate samples in all to produce the data analyzed in this study.
Article Snippet: The following pairs of human tumor and adjacent normal 5 μm tissue sections from single
Techniques: Microscopy
Journal: Nature Communications
Article Title: High resolution mapping of the tumor microenvironment using integrated single-cell, spatial and in situ analysis
doi: 10.1038/s41467-023-43458-x
Figure Lengend Snippet: a Xenium spatial plot for ERBB2 (HER2—gray), ESR1 (estrogen receptor—green), and PGR (progesterone receptor—magenta) decoded transcripts. Scale bar = 1 mm. b Closer view of triple-positive ROI. Scale bar = 0.2 mm. c Corresponding H&E image. d Cell types contained within ROI reveal that this is a DCIS #2 tumor epithelium. e Individual Xenium spatial plots from ( b ). f Chromium scFFPE-seq yields only about 30 cells that are positive for PGR , but these cells do not express ERBB2 or ESR1 . g Triple-positive region is identified in Visium (given a priori knowledge from Xenium) and is h part of a distinct cluster (see Fig. ). i Spot interpolation (see Supp. Fig. ) provides cell type frequencies within each Visium spot. Color code legend is shown in ( d ). j Visium H&E and four representative differentially expressed genes in the tumor epithelium (94 genes; log 2 FC > 1.5; p -value < 0.05) revealed by Visium data across the whole transcriptome. Scale bar = 1 mm. Differential expression was performed in Loupe Browser (see “Methods”), which performs a variant of the negative binomial exact test (for small gene counts), or a fast asymptotic beta test derived from edgeR (for large gene counts). P -values were adjusted for multiple testing using the Benjamini–Hochberg procedure to control for the false discovery rate. Both the Xenium and Visium experiments were performed in replicate on two serial sections, with one representative section from each technology shown here.
Article Snippet: A single formalin-fixed, paraffin-embedded (
Techniques: Expressing, Variant Assay, Derivative Assay
Journal: Nature Communications
Article Title: High resolution mapping of the tumor microenvironment using integrated single-cell, spatial and in situ analysis
doi: 10.1038/s41467-023-43458-x
Figure Lengend Snippet: Antibodies used for the post-Xenium IF staining
Article Snippet: A single formalin-fixed, paraffin-embedded (
Techniques: